Bologna coach Inzaghi: Emotional facing Ancelottiby Carlos Volcano10 months agoSend to a friendShare the loveBologna coach Filippo Inzaghi is looking forward to facing Carlo Ancelotti’s Napoli.Inzaghi played for Ancelotti at AC Milan.”Facing Carlo will be really emotional,” Inzaghi said in his pre-match Press conference.“He’s a great man and we have a strong and emotional bond. We’ve always had a wonderful relationship.“However, I hope I can make him feel sad, after all the joy I brought him throughout my career…” About the authorCarlos VolcanoShare the loveHave your say
MINNEAPOLIS — The Latest on EPA criticism of a key permit for the planned PolyMet mine in Minnesota (all times local):3:55 p.m.Minnesota regulators are defending their process for approving a key permit for the planned PolyMet copper-nickel mine.Newly released documents show that staffers with the federal Environmental Protection Agency criticized how the Minnesota Pollution Control Agency drafted the water quality permit, and concluded that the permit would violate federal law because it lacked pollution limits based on the state’s water quality standards.But MPCA spokesman Darin Broton says the permitting process was rigorous. He says the MPCA and EPA had frequent conversations during the entire permitting process. Based on those conversations, he says, as well as other comments received from the public during the official comment period, the MPCA made substantive changes to the draft permit. He says that’s why the EPA did not object to the MPCA’s final permit.____1:30 p.m.Environmental Protection Agency documents show that its staffers were critical of how Minnesota regulators drafted a key permit for the planned PolyMet copper-nickel mine. And they show the officials concluded the permit would violate federal law because it lacked specific water pollution limits.The EPA released the documents after a court challenge by WaterLegacy and other groups.WaterLegacy attorney Paula Maccabee says that while the EPA had serious concerns, they weren’t reflected in PolyMet’s final permit, which did not set limits for specific pollutants.EPA staffers read one document over the phone to staff at the Minnesota Pollution Control Agency last year but never filed formal written comments expressing concerns.The Minnesota agency said it was preparing a statement. EPA officials did not immediately respond to a request for comment.The Associated Press
According to the proposed draft on the programme of work of the Constitutional Assembly to be presented to Parliament next month, in the absence of the Speaker, the Constitutional Assembly shall elect one of the Deputy Chairmen to chair the sittings of the Assembly. The proposed Constitutional Assembly to be chaired by the Speaker of Parliament will have seven Deputy Chairmen.The seven Deputy Chairmen of the Constitutional Assembly will be elected by the Constitutional Assembly. Sub-Committees, consisting of Members of the Constitutional Assembly, can also be appointed by the Constitutional Assembly provided that each such Sub-Committee shall comprise of not more thaneleven (11) Members. The Chairman of each Sub-Committee will be appointed by the Steering Committee.According to the proposal, the Prime Minister will, at the first meeting of the Constitutional Assembly, present a Resolution for adoption by the Constitutional Assembly, calling upon the Steering Committee to present a Resolution proposing a Draft Constitution for the consideration of the Constitutional Assembly, prior to its submission to the Cabinet of Ministers and Parliament. (Colombo Gazette) A Steering Committee consisting of the Prime Minister (Chairman), Leader of the House of Parliament, Leader of the Opposition, the Minister of Justice, and not more than seventeen (17) other Members of the Constitutional Assembly will be appointed by the Constitutional Assembly.The Steering Committee shall be responsible for the business of the Constitutional Assembly and for preparing a Draft of a new Constitution for Sri Lanka. “The Prime Minister shall, at the first meeting of the Constitutional Assembly, present a Resolution for adoption by the Constitutional Assembly, calling upon the Steering Committee to present a Resolution proposing a Draft Constitution for the consideration of the Constitutional Assembly, prior to its submission to the Cabinet of Ministers and Parliament,” the draft presented to the order book of Parliament states.
“Development goals should shape the framework of globalization rather than allowing the blind forces of globalization to define the outcome of development,” the Secretary-General writes in the report on the UN’s role in promoting development.He notes that his views and recommendations should be seen in the context of two events that will provide major opportunities to address many of the core issues that arise from the dynamic interaction of trade, finance, technology and investment – the World Trade Organization (WTO) ministerial meeting, scheduled for next month in Doha, Qatar, and the International Conference on Financing for Development to be held in early 2002 in Mexico. Citing recent assessments, the report suggests a substantial decline in global economic growth, with industrial production and business and consumer confidence falling in many developed countries. Practically every forecast made towards the first half of 2001 had to be modified downward in the year’s third quarter without even taking into account the potential effect of the 11 September terrorist attacks on the global economy. The attacks’ precise impact could depend on how trade, business and consumer confidence, investment and capital flows will be affected, the report says, pointing out that certain industries – most notably travel and tourism – are already experiencing a direct impact. “Events over the forthcoming weeks will therefore be essential for assessing the direction in which the world economy is moving,” Mr. Annan says.Given its universality and broad mandate, the UN “has an important role to play in promoting policy coherence with the explicit goal of placing development and poverty eradication at the centre of policies and processes related to global integration,” the Secretary-General says.”There is an urgent need,” he writes, “for a partnership encompassing the Governments of both developing and developed countries, international organizations, civil society and the private sector in order to ensure the adequate flow of resources, knowledge and technology to developing countries so that they can effectively address the underlying causes which stand as obstacles to sustainable development and poverty eradication.”
United Nations staff are gradually returning to the troubled city of Heart in western Afghanistan, where the offices of the UN mission and agencies and non-governmental organizations (NGOs) were ransacked and seriously damaged on Sunday, to try to restore the world body’s operations there.A new UN compound should be ready for operations within a week, a spokesman for the UN Assistance Mission in Afghanistan (UNAMA) told reporters today at a press briefing in the capital Kabul.Manoel de Almeida e Silva said 43 staff have returned to Herat in the past two days to join the 15 international staff and hundreds of Afghan personnel who stayed on in the city after Sunday’s wave of violence.The returning UN workers, who include engineers, will help install 20 to 25 portable units that can be used both as offices and as staff accommodation. The units have satellite and Internet communications, and also contain bedding, furniture and generators. The World Food Programme (WFP) has also resumed its operations to Heart, sending a convoy of trucks carrying 170 tons of food to the city from the neighbouring province of Ghor to alleviate the suffering caused by the long-running drought in the west. But Mr. de Almeida e Silva said the UN staff in Heart are continuing “to proceed with caution and prudence” given that the offices or compounds of UNAMA, the UN High Commissioner for Refugees (UNHCR), the UN Children’s Fund (UNICEF) and the World Health Organization (WHO) were all targeted. Local police say they have recovered three UN cars and 68 other items belonging to the world body. Meanwhile, hundreds of returning Afghan refugees who were stranded on the Iranian side of the border near Herat since the weekend’s violence forced UNHCR to halt operations have now resumed their journey home. Some 1,627 Afghans, travelling in a convoy of buses, crossed the border this morning and later passed through Herat without incident, UNHCR reported.
IOWA CITY, Iowa — Nebraska fired coach Bo Pelini in part because beating Iowa wasn’t good enough. The Huskers canned his replacement, Mike Riley in part because they weren’t good enough to beat the Hawkeyes.New coach Scott Frost will get his first crack at Iowa on Friday in a border rivalry that’s blossomed into something of annual referendum on both programs.The Hawkeyes (7-4, 4-4 Big Ten) snapped a three-game losing streak last weekend with a cathartic 63-0 whitewash of Illinois on the road. Nebraska (4-7, 3-5) has won four of its last five after a 0-6 start and would like nothing more than to enter the off-season with a win at Iowa.The Hawkeyes have won three straight over the Huskers, including a 56-14 blowout in Lincoln a year ago that helped seal Riley’s fate.“This game is extremely important to us,” Iowa coach Kirk Ferentz said. “It’s got a lot of weight, a lot of value, and we’re giving it our utmost intention … You look at Nebraska, they’re a team that’s really riding high right now, playing with really good momentum.”Iowa’s seniors will try to make it a clean sweep of Nebraska, which has lost the last two in the series by 30 and 42 points.IOWA’S ATTACKAfter losing to Penn State, Purdue and Northwestern by just 12 combined points, the Hawkeyes exploded for nine touchdowns against the Illini. Iowa scored in nearly way imaginable, racking up touchdowns on runs, passes, a fumble return and a punt return.“I’ve certainly watched them enough to know that they’re really good on offence, I’ve been impressed. I think they’re more multiple than they used to be,” Frost said.QB MARTINEZThe Hawkeyes know that they will have to be wary of Nebraska quarterback Adrian Martinez. He needs just 90 yards of total offence to become the first freshman in school history with 3,000.“He’s not (Penn State quarterback Trace) McSorley, but there are probably some parallels in there,” Ferentz said. “He’s really a very threatening guy, running the football, throwing the football. They’ve got designed runs, designed options, those types of things, and then also he’ll pull it down and go.”IOWA’S DIowa has pitched a pair of shutouts, and the Hawkeyes held four other opponents to 14 points or less. The Hawkeyes rank ninth nationally in scoring defence at 16.5 points per game and will likely be the toughest unit Nebraska has seen outside of Michigan, which beat the Huskers 56-10.“They are another tough, solid team,” Martinez said. “They are going to be very sound and play hard. We are going to have to come to play there. They are not going to make a lot of mistakes.”THE NUMBERSLast week’s win was the 150th for Ferentz at Iowa. Ferentz also won 12 times at Maine. … Nebraska leads the series 10-7 in games played in Iowa City. … Iowa leads the Big Ten with 17 interceptions. … Nebraska’s Stanley Morgan Jr. needs just 24 receiving yards to set the school’s career record. … The Hawkeyes are 12-3 in their last 15 trophy games. All three losses in that span came against Wisconsin.HE SAID IT (JOKINGLY)“His sustained success is impressive, but you still don’t get to 150 unless you’re a little bit old. I’ll make sure I remind him of that when I see him.” — Frost about Ferentz.___More AP college football: https://apnews.com/Collegefootball and https://twitter.com/AP_Top25Luke Meredith, The Associated Press
A 29-year-old man has been charged with grievous bodily harm and possession of a knife after a civil servant in his 60s was stabbed on Marsham Street, outside the Home Office, on Thursday.Dominic Hornberger, 29, who is from Birmingham, was charged on Friday and appeared at Westminster Magistrates’ Court on Saturday, Scotland Yard said.He was remanded in custody to appear at Southwark Crown Court on Friday September 13.Photographs from the scene showed the alleged victim, who has not been named and works for the Ministry of Housing, Communities and Local Government, shirtless with blood pouring from his face.He was taken away in an ambulance. While his injuries were first treated as life-threatening by paramedics, police later said they were less serious than first thought. Want the best of The Telegraph direct to your email and WhatsApp? Sign up to our free twice-daily Front Page newsletter and new audio briefings.
Recognised “for their outstanding performance in the Towards Sustainable Mining (TSM) initiative and commitments to corporate responsibility”, the Mining Association of Canada (MAC) has presented eight mining facilities operated by Agnico Eagle Mines Ltd, First Quantum Minerals Ltd, IAMGOLD Corporation and Teck Resources Ltd with TSM Leadership Awards. This year, for the first time, TSM Leadership Awards were given to mining operations outside of Canada. These include Agnico Eagle’s Kittila mine in Finland, First Quantum Minerals’ Çayeli mine in Turkey, IAMGOLD’s Essakane gold mine in Burkina Faso and IAMGOLD’s Rosebel gold mine in Suriname.Implementation of TSM is mandatory for all MAC members’ Canadian facilities, however, these companies voluntarily apply TSM to their international sites as a way of demonstrating that they are managing their responsibilities throughout the world to the same high standard as their Canadian operations. “This year, we recognise eight facilities with TSM Leadership Awards for their stand-out performance in our TSM initiative and congratulate them and their staff for raising the bar in environmental and social performance. Awarded annually, this is the highest number of winners we have seen yet,” said Pierre Gratton, President and CEO, MAC.The 2015 TSM Leadership Award Winners are as follows:• Agnico Eagle Mines Ltd, Goldex mine• Agnico Eagle Mines Ltd, Kittila mine• Agnico Eagle Mines Ltd, LaRonde mine• First Quantum Minerals Ltd, Çayeli mine• IAMGOLD Corporation, Essakane mine• IAMGOLD Corporation, Rosebel mine• IAMGOLD Corporation, Westwood mine• Teck Resources Ltd, Line Creek OperationsTSM’s requirements go well beyond regulatory obligations and are designed to improve industry’s operational performance in key environmental and social areas of mining. The TSM Leadership Award is granted only when an operation meets or exceeds a Level “A” ranking for all 23 indicators under the six performance areas of the TSM initiative (known as “protocols”) – safety and health, Aboriginal and community outreach, crisis management, tailings management, biodiversity conservation management, and energy use and greenhouse gas emissions management. An operation’s TSM results must be externally verified to be eligible for this recognition. The 2015 winners were selected based on their 2014 TSM performance results.
Share1589 Tweet Email1 A DOG THAT was left in a hot car with windows closed in yesterday’s heat was found to be ‘in distress, uncomfortable and breathing heavily’.A spokesperson for DSPCA told TheJournal.ie that the small dog was discovered in a car in Dublin 4 with an empty bowl of water and no ventilation or shade.They said the dog was locked in the car with all the windows closed for an hour before the owner returned.DSPCA inspectors and gardai were about to break the car windows when the owner returned to his vehicle.The DSPCA added that further investigations are ongoing.Advice for keeping animals coolThe organisation is also reminding people that the hot weather ahead can be uncomfortable and dangerous for pets. Education officer for the DSPCA Gillian Bird said: File Photo Image: Shutterstock/Jarhe Photography Gardaí and DSPCA warn dog owners to be careful after dog left in hot car found ‘in distress’ DSPCA inspectors and gardai were about to break the car windows when the owner returned to his vehicle. 28 Comments http://jrnl.ie/4095832 Jun 28th 2018, 6:16 AM People know that dogs should not be left in cars for even a few minutes in weather this hot, but other pets have to be taken into consideration on days like today.Here are a few helpful hints to keep pets safe and comfortable today:Keep pets like dogs and cats indoors in a cool room with plenty of fresh water. Pets often lie out in the sun for longer than is good for themMake sure that the sun cannot shine in to the room by pulling curtains and keeping windows open enough to get a good breezeDon’t overfeed them, on hot days too much food can upset the stomach and make them uncomfortablePop some wet dog or cat food into an empty yogurt pot and freeze it, it will make a nice cooling ice pop for them to enjoyWet a towel and put it for your dog to lie on, it will help him keep coolMake sure pets such as rabbits, fish and pet birds are out of the direct sunlight and have plenty of shade to keep coolBe careful of walking your dog as the paths can get very hot in the sun and can burn their paws, check the temperature with your hand or feet or wait until later in the evening before exercising themIf you have any concerns that your pet has gotten too hot and is showing signs of heat exhaustion contact your vet for adviceFinally, do not take your dog in the car if it can be avoided, even for a short journey. Even with the windows open it can be too hot and your pet will suffer. Image: Shutterstock/Jarhe Photography Short URL File Photo By Cliodhna Russell Thursday 28 Jun 2018, 6:15 AM 21,409 Views Tweet thisShare on FacebookEmail this article
March began with tributes to Greek women and ended with the death of Archibishop Stylianos. In between we had Greek Independence Day parades and controversies, including tragedies in Christchurch.Greek Independence Day was celebrated around Australia, however there were a few glitches in Melbourne as people yelled in protest against the visit by Greek Parliamentary Deputy George Varemenos. While the right to protest was not an issue, there was debate as to whether such forms of protest should be made at sacred areas such as the Shrine of Remembrance.Following Bill Shorten’s visit to the Lonsdale Street Greek Festival last month, Australian Prime Minister Scott Morrison visited the Greek Centre where he danced tsamikos and met with the Greek Community.Shortly after Mr Morrison’s visit, it was announced that president of the Greek Orthodox Community of Melbourne and Victoria, Bill Papastergiadis would run as an independent in the next federal elections. Archbishop Stylianos’ coffin leaving the Cathedral of the Annunciation of Our Lady and being taken to Rookwood Cemetery. Photo: Nick Bourdo PhotographyGreek Deputy Foreign Affairs Minister Terens Quick poses with a Greek Australian student during his visit with the diaspora as part of NOSTOS III. Photo: K. DevesSt Johns College student, Evangelos Athanasiou prepares for Greek Independence Day. Photo: SuppliedPhoto: SBSThe Sydney parade for Greek Independence Day culminated with celebrations at the steps of Sydney Opera House. St Paisios Church is nearing completion in Adelaide.Even those of us who aren’t survivors of breast cancer know someone who has suffered, whether this be a friend, mother, daughter. For this reason, women from all walks of life who gathered at the International Women’s Day charity luncheon organised by the Lemnian Community of Victoria’s Ladies Committee laughed, shed a tear and got a chance to be informed about breast cancer.Guests at the Merrimu Reception Hall numbered 200 women, who had the opportunity to ponder the disease and how it has developed through time, while bonding over good food and great company.They enjoyed talks by investment banker-cum-chef/cook book writer Kathy Tsaples and Dr Helen Kavnoudias that provided food for thought and messages of hope. Photo: SuppliedThe Christchurch attack may have struck at two mosques, however people from all walks of life came together to support the victims and their family.Soon after the attack, Dimitris Greek Food closed for the evening in order to help donate and deliver platters of food to families of victims waiting at the hospital.PM Scott Morrison danced tsamiko at the Greek centre. These two cuties flew their kites on Clean Monday.Greek women’s volleyball team, Vicky Arvaniti and Penny Karagkouni, came fifth at the FIVB World Tour in Sydney.Representative Katerine Duska Released her video clip for Eurovision. Lots of fun at the Kolyva-making master class at Altona. Eleonora Zouganelli headed to the Greek Festival of Sydney following her performance at the Lonsdale Street Greek Festival.Greek Festival in Sydney was a huge success at Darling Harbour.On Saturday 23 March, the Pammessinian Brotherhood Papaflessas celebrated its 60 year anniversary at Merrimu Receptions.. Photo: K. DevesHRH The Prince of Wales hosts a reception for The Prince’s Trust International in the State Rooms of St James’s Palace, London.Photo by Ian JonesThe Greek Rebetiko Festival was a huge success. Archbishop Stylianos’ coffin leaving the Cathedral of the Annunciation of Our Lady and being taken to Rookwood Cemetery. Photo: Nick Bourdo Photography Facebook Twitter: @NeosKosmos Instagram
NEW YORK — Sears filed for Chapter 11 bankruptcy protection Monday, with plans to shutter 142 unprofitable stores in the hopes that it can stay in business.The question now is whether a smaller version of the company that once towered over the American retail landscape can be viable. Sears, which started as a mail order catalog in the 1880s, has been on a slow march toward extinction as it lagged far behind its peers and incurred huge losses over the years.“This is a company that in the 1950s stood like a colossus over the American retail landscape,” said Craig Johnson, president of Customer Growth Partners, a retail consultancy. “Hopefully, a smaller new Sears will be healthier.”Others don’t share Johnson’s optimism.“That a storied retailer, once at the pinnacle of the industry, should collapse in such a shabby state of disarray is both terrible and scandalous in equal measure,” said Neil Saunders, managing director of GlobalData Retail, in a note published Monday. “In our view, too much rot has set in at Sears to make it viable business.”The company has struggled with outdated stores and complaints about customer service even for its once crown jewels: major appliances like washers and dryers. That’s in contrast with chains like Walmart, Target, Best Buy and Macy’s, which have been enjoying stronger sales as they benefit from a robust economy and efforts to make the shopping experience more inviting by investing heavily in remodeling and de-cluttering their stores.
Roberto Carlos recently admitted Philippe Coutinho is blooming, even though he is still under Neymar’s shadow, and might prove a key player for Brazil in the World Cup.Despite the fact that the Barcelona star scored twice in Brazil’s three games in Russia and Neymar did so only once, Neymar is still under the spotlight of the global media.Roberto Carlos, who won the World Cup in 2002, thinks if that continues, Coutinho can easily keep up the good work and even step out of the shadow.“Philippe is in a very good moment, in Barcelona and in the Brazilian team,” Carlos said, according to Sportskeeda.Neymar responds to PSG criticism with a stunning winner Tomás Pavel Ibarra Meda – September 14, 2019 Despite all the backlash he got today at Parc des Princes, Neymar responded by scoring a stunning winner vs Strasbourg.We all knew that Neymar’s…“He’s a very important player for Brazil, because the pressure is all on Neymar, then Coutinho shows up. The Brazilian team have a very strong chance to win this World Cup.”“Neymar is gettting better. He’s relaxed. He’s recovered well from his injury. He’s a very important player for Brazil. He is very calm, that is the most important thing. When he is calm I know that he can offer much more.”He also went on to advise the Brazilians to not underestimate Mexico and there is no easy games from the upcoming ones as well.
Copyright 2019 Sunbeam Television Corp. All rights reserved. This material may not be published, broadcast, rewritten or redistributed. MIAMI (WSVN) – Rescue crews rushed a male victim, possibly a juvenile, to the hospital after, officials said, he was shot in a Miami neighborhood, Sunday evening.City of Miami Police and Fire Rescue units responded to the scene of the shooting near Northwest 10th Avenue and 47th Terrace.Investigators said the victim was riding his bicycle when an unknown vehicle approached him. Someone from inside the vehicle then opened fire, striking the victim several times.Area residents rendered aid to the victim before rescuers arrived, bringing him towels to help stop the bleeding.“I was shocked. It was like, I’m panicking,” said neighbor Trovaris Paris. “When I was trying to speak to him, he was out of it. That’s why, after that, I was trying to make the police, like, ‘Help him, help him, help him out.’ That’s when I guess they put him behind the rescue’s thing, and I guess that’s when they took him on.”Paramedics transported the victim to Ryder Trauma Center in critical condition.Officials have not specified his age but said he looks younger than 20 years old.
(Graphic courtesy of NOAA)The potential for a La Nina weather pattern this winter is looking more and more likely. The latest forecast says there’s a 66 percent chance for La Nina, a figure that’s been steadily rising for the last few months.That’s according to Brian Brettschneider, with our Ask a Climatologist segment.He said La Nina typically brings cooler and drier conditions to Alaska. And because of global warming, that may mean a more typical winter.Listen nowInterview Transcript:Brian: Since we’re comparing against the 1981 to 2010 climate normal period, we’re already a couple of degrees warmer than that. So we’re starting off, all things being equal, as being pretty far above normal. La Nina, and it’s not a done deal, generally it’s going to tilt us toward cooler temperatures. So more than likely it’s going to send us back a couple of degrees, which would put us close to the normal range.Annie: We had a La Nina last winter. How unusual is it to have back-to-back La Nina’s?Brian: It is unusual, but it’s not unprecedented. It’s not one La Nina- we had a La Nina last winter and it faded away, but now we’re transitioning back into a new La Nina, so they are separate events. Again, it’s not unprecedented, but it is pretty uncommon. Normally, they’re going to be spaced a couple years apart.Annie: Do we know why we would have two in a row?Brian: Actually, we don’t. With El Nino’s and La Nina’s, we kind of understand how they work, but we don’t have a good understanding of what initiates them or why they occur. So that’s an area of intense research. But for now, we watch the progression of it and don’t understand why they occur.Annie: How does the La Nina work and why does it bring drier, cooler weather to Alaska?Brian: El Ninos and La Nina’s- they’re a measure of what the ocean temperatures are in an area of the central tropical Pacific Ocean within five degrees of the equator. And it’s curious as to how that affects things in Alaska. The reason it does is because it’s where these warm pools of water set up. In El Nino, they set up more towards South America. In La Nina years, they set up much more towards the west, say towards Indonesia. So you have these tropical thunderstorms sending heat, air movement, masses of air, and that affects large scale atmospheric patterns, called Walker Cells. And it also influences which direction the wind is coming from at upper levels. So in that La Nina year where the warm pool is set up to the west, and that’s where the tropical storms are, we end up with a kind of northerly flow across Alaska which drags in colder air from farther to the north and limits the amount of moisture that comes in.
(Phys.org)—A team of researchers at the University of Washington has created microscopic assemblies for packaging genetic material that they call synthetic nucleocapsids. The team hopes the assemblies can one day be used to treat patients with cellular-level problems by delivering appropriate therapies to the cells that could benefit from them. In their paper published in the journal Nature, the team describes their assemblies and what they have done with them thus far. A hairpin loop from a pre-mRNA. Highlighted are the nucleobases (green) and the ribose-phosphate backbone (blue). Note that this is a single strand of RNA that folds back upon itself. Credit: Vossman/ Wikipedia © 2017 Phys.org Explore further Citation: A new way to deliver mRNA genomes: Nucleocapsids with evolutionary properties (2017, December 14) retrieved 18 August 2019 from https://phys.org/news/2017-12-mrna-genomes-nucleocapsids-evolutionary-properties.html More information: Gabriel L. Butterfield et al. Evolution of a designed protein assembly encapsulating its own RNA genome, Nature (2017). DOI: 10.1038/nature25157AbstractThe challenges of evolution in a complex biochemical environment, coupling genotype to phenotype and protecting the genetic material, are solved elegantly in biological systems by the encapsulation of nucleic acids. In the simplest examples, viruses use capsids to surround their genomes. Although these naturally occurring systems have been modified to change their tropism and to display proteins or peptides, billions of years of evolution have favoured efficiency at the expense of modularity, making viral capsids difficult to engineer. Synthetic systems composed of non-viral proteins could provide a ‘blank slate’ to evolve desired properties for drug delivery and other biomedical applications, while avoiding the safety risks and engineering challenges associated with viruses. Here we create synthetic nucleocapsids, which are computationally designed icosahedral protein assemblies with positively charged inner surfaces that can package their own full-length mRNA genomes. We explore the ability of these nucleocapsids to evolve virus-like properties by generating diversified populations using Escherichia coli as an expression host. Several generations of evolution resulted in markedly improved genome packaging (more than 133-fold), stability in blood (from less than 3.7% to 71% of packaged RNA protected after 6 hours of treatment), and in vivo circulation time (from less than 5 minutes to approximately 4.5 hours). The resulting synthetic nucleocapsids package one full-length RNA genome for every 11 icosahedral assemblies, similar to the best recombinant adeno-associated virus vectors. Our results show that there are simple evolutionary paths through which protein assemblies can acquire virus-like genome packaging and protection. Considerable effort has been directed at ‘top-down’ modification of viruses to be safe and effective for drug delivery and vaccine applications; the ability to design synthetic nanomaterials computationally and to optimize them through evolution now enables a complementary ‘bottom-up’ approach with considerable advantages in programmability and control.Press release The development of the assemblies was part of an overall program dedicated to mRNA delivery. mRNA, the team explains, are the molecules that cells use to send messages between DNA (the blueprint) and the proteins that reside in the cells. The idea for the nucleocapsids came from viruses, the group further explains, which encapsulate their payloads as a means of protecting them. In designing their own delivery system, the researchers used proteins to build assemblies containing their own RNA genome. The assemblies can evolve indirectly, the team notes—assemblies are created and then injected into test mice. After a period of time has elapsed, blood is drawn from the test mice and examined to see how well the assembly delivered its load to targeted cells. Changes can then be made to improve desirable characteristics. The researchers note that the technique allows for large leaps in improvement after just a few iterations. They found, for example, that they could improve the survival rate of an RNA package injected into a mouse after six hours from 3.7 percent to 71 percent. They also found that they could slow circulation time inside of a mouse, from five minutes to four and a half hours, giving an assembly more time to do its job.The will continue to work with the synthetic nucleocapsids, looking for ways not only to improve performance, but to allow for targeting specific types of cells and carrying a wider range of payloads—including RNA, DNA, and proteins and small-molecule drugs. They are hoping the end product will be a delivery system that can be used to battle cancer, heart disease, and other ailments that involve problems with cells. Journal information: Nature This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. Designer proteins that package genetic material could help deliver gene therapy
Click on the words highlighted in red to read more on this and related topics.If you are reading this on your cellphone and there are telephone numbers provided in the text, you can call these simply by clicking on them.To receive news links via WhatsApp.For the latest news, visit our webpage or follow us on Facebook and Twitter. Join us there! WebsiteWebsiteWebsite WebsiteWebsiteWebsite WebsiteWebsiteWebsite 2 light motor vehicles were involved in a collision this afternoon in Murchison Street.Public Safety, Towing Services and other emergency personnel were on scene.There were no injuries.It took officials less than half an hour to clear the scene.DID YOU KNOW?
THAI sponsors Visit Japan FIT Travel Fair 2017THAI sponsors Visit Japan FIT Travel Fair 2017Thai Airways International Public Company Limited (THAI), recently sponsored “Visit Japan F.I.T. Travel Fair 2017”, which was organized by Japan National Tourism Organization (JNTO) at Royal Paragon Hall 3, Siam Paragon. Mr. Kazuhiro Ito (fourth from left), JNTO Executive Director, Bangkok Office, presided over the opening ceremony, which was attended by Mr. Korakot Chatasingha (third from left), THAI Director of Thailand Regional Department. At the event, THAI offered special fares on various routes from Thailand to Japan, those being Narita, Haneda, Osaka, Nagoya, Fukuoka, and Sapporo.Source = Thai Airways International Public Company Limited (THAI)
Niagara Falls, Ontario, is a Canadian city at the famous waterfalls of the same name, which is linked with the U.S. by the Rainbow Bridge. Its site on the Niagara River’s western shore overlooks the Horseshoe Falls, the cascades’ most expansive section. Source: Expedia
Share June 2, 2016 574 Views in Daily Dose, Data, Government, Headlines, Market Studies, News “Participants agreed that their ongoing assessments of the data and other incoming information, as well as the implications for the outlook, would determine the timing and pace of future adjustments to the stance of monetary policy,” the minutes from the FOMC meeting said. “Most participants judged that if incoming data were consistent with economic growth picking up in the second quarter, labor market conditions continuing to strengthen, and inflation making progress toward the Committee’s 2 percent objective, then it likely would be appropriate for the Committee to increase the target range for the federal funds rate in June.” This week, mortgage rates trended up while applications declined as the Federal Reserve sent out large signals that a rate hike is imminent in June, but Friday’s employment report could completely derail those plans.Freddie Mac’s Primary Mortgage Market Survey showed that average fixed mortgage rates moved up slightly from last week but are still near three-year lows.According to the report, the 30-year fixed-rate mortgage (FRM) averaged 3.66 percent with an average 0.5 point for the week ending June 2, 2016. This rate is up from last week when it averaged 3.64 percent but down from year ago at this time when the 30-year FRM averaged 3.87 percent.Sean Becketti, Chief Economist at Freddie Mac said, “Since jumping 11 basis points on May 18th, the 10-year Treasury yield has leveled-off around 1.85 percent. Mortgage rates continue to adjust to this new level with the 30-year fixed rate inching up another 2 basis points this week to 3.66 percent. Recent statements by the Fed appear to have persuaded the market that a rate hike may come sooner than later. However, the market is fickle, and Friday’s employment report has the potential to swing opinion 180 degrees in the other direction.”The 15-year FRM averaged 2.92 percent this week with an average 0.5 point, up from last week when it averaged 2.89 percent. A year ago at this time, the 15-year FRM averaged 3.08 percent.As mortgage rates moved up, buyers fled from the market as mortgage applications decreased 4.1 percent for the week ending May 27, 2016, according to the Mortgage Bankers Association’s (MBA) Weekly Mortgage Applications Survey.The MBA reported that on an unadjusted basis, the Index fell 5 percent compared with the previous week. The Refinance Index decreased 4 percent from the previous week, while the seasonally adjusted Purchase Index decreased 5 percent from one week earlier. The government purchase index decreased 6 percent from one week earlier to the lowest level since November 2015. The unadjusted Purchase Index decreased 6 percent compared with the previous week and was 28 percent higher than the same week one year ago.Refinance applications increased to 54.3 percent of total applications from 53.7 percent the previous week, while the adjustable-rate mortgage (ARM) share of activity decreased to 5.0 percent of total applications.The FHA share of total applications decreased to 12.5 percent from 12.7 percent the week prior, the report said. The VA share of total applications increased to 12.0 percent from 11.5 percent the week prior, and the USDA share of total applications remained unchanged from 0.7 percent the week prior.Jonathan Smoke, Chief Economist of Realtor.com said, “Given weaker economic growth in the first quarter and lingering global economic concerns, the Federal Reserve already seemed unlikely to push aggressive increases in short-term rates this year. After last Friday’s report on April’s weaker level of job creation, the odds of the Fed raising rates in June diminished even further. The direction for rates for the rest of the summer and into the future remains murky. I’ve surveyed a range of recent forecasts from the Mortgage Bankers Association, Freddie Mac, Fannie Mae, and highly respected macro economists, and I found quite a bit of divergence.”It may be sooner; Fed officials have dropped strong hints that a June rate hike is forthcoming. Fed governor Jerome Powell, a voting member of FOMC, said in a public address recently that he believes the economy is on “solid footing” and that the economy could be ready for the Fed to raise the federal funds target rate “fairly soon.” After June, the next FOMC meeting will be July 26-27. Federal Reserve Mortgage Applications Mortgage Rates Rate Hike 2016-06-02 Staff Writer Mortgage Round-Up: Rates, Applications Move in Opposite Directions
With the perfect synergy of history and high fashion, the world’s most legendary diamond is slated for a makeover from one of the world’s most famous jewelers.Over half a century ago, Hollywood favorite Harry Winston purchased the Hope diamond—widely renowned as the largest deep blue diamond in the world—from the estate of American socialite Evalyn Walsh McLean. After a nationwide tour with the 45.52-carat Hope as part of his famous “Court of Jewels”, Winston donated the diamond to the Smithsonian Institution National Museum of Natural History in Washington, D.C. as a “gift to the American people”—reportedly sending it, astonishingly, through the U.S. postal service.To honor the 50th anniversary of Winston’s most generous bequest, the American jeweler is giving the diamond a special redesign. The House of Harry Winston has prepared three unique settings, each one symbolizing modern hope in America. The public will be able to vote for its favorite design online from August 19th until September 7th, and the winning setting will be announced during a press conference in Washington, D.C., in conjunction with the world premiere of the Smithsonian Channel’s “Mystery of the Hope Diamond”, scheduled to air in March 2010. The Hope’s new setting will be unveiled and televised nationally.Visit www.smithsonianchannel.com/hope for more information and to cast your vote.